Construct: shRNA TRCN0000293214

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144570.2-590s21c1
DNA Barcode:
CCAGCATCTCCTTCTACTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
JPT2 (90861)
Gene Description:
Jupiter microtubule associated homolog 2
Transcript:
RefSeq NM_144570.2 (NON-CURRENT)
Match location:
Position 590 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293214)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCATCTCCTTCTACTAAG
Hairpin Sequence:
5'-CCGG-CCAGCATCTCCTTCTACTAAG-CTCGAG-CTTAGTAGAAGGAGATGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCATCTCCTTCTACTAAGCTCGAGCTTAGTAGAAGGAGATGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCATCTCCTTCTACTAAGCTCGAGCTTAGTAGAAGGAGATGCTGG-3'

Other clones with same target sequence:

(none)