Construct: shRNA TRCN0000293216

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003664.3-2765s21c1
DNA Barcode:
AGGACTAGCTGCCCATTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP3B1 (8546)
Gene Description:
adaptor related protein complex 3 subunit beta 1
Transcript:
RefSeq NM_003664.3 (NON-CURRENT)
Match location:
Position 2765 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293216)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGACTAGCTGCCCATTATTT
Hairpin Sequence:
5'-CCGG-AGGACTAGCTGCCCATTATTT-CTCGAG-AAATAATGGGCAGCTAGTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGACTAGCTGCCCATTATTTCTCGAGAAATAATGGGCAGCTAGTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGACTAGCTGCCCATTATTTCTCGAGAAATAATGGGCAGCTAGTCCT-3'

Other clones with same target sequence:

(none)