Construct: shRNA TRCN0000293236

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006432.3-251s21c1
DNA Barcode:
GCTGAGCAAAGGACAGTCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPC2 (10577)
Gene Description:
NPC intracellular cholesterol transporter 2
Transcript:
RefSeq NM_006432.3 (NON-CURRENT)
Match location:
Position 251 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293236)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAGCAAAGGACAGTCTTA
Hairpin Sequence:
5'-CCGG-GCTGAGCAAAGGACAGTCTTA-CTCGAG-TAAGACTGTCCTTTGCTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGCAAAGGACAGTCTTACTCGAGTAAGACTGTCCTTTGCTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGCAAAGGACAGTCTTACTCGAGTAAGACTGTCCTTTGCTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000029321