Construct: shRNA TRCN0000293284

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031370.2-1295s21c1
DNA Barcode:
ACTATGGATATGGTGATTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HNRNPD (3184)
Gene Description:
heterogeneous nuclear ribonucleoprotein D
Transcript:
RefSeq NM_031370.2 (NON-CURRENT)
Match location:
Position 1295 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293284)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTATGGATATGGTGATTATA
Hairpin Sequence:
5'-CCGG-ACTATGGATATGGTGATTATA-CTCGAG-TATAATCACCATATCCATAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTATGGATATGGTGATTATACTCGAGTATAATCACCATATCCATAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTATGGATATGGTGATTATACTCGAGTATAATCACCATATCCATAGT-3'

Other clones with same target sequence:

(none)