Construct: shRNA TRCN0000293439

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012164.3-764s21c1
DNA Barcode:
AGCACAGGGCAGTGCGTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXW2 (26190)
Gene Description:
F-box and WD repeat domain containing 2
Transcript:
RefSeq NM_012164.3 (NON-CURRENT)
Match location:
Position 764 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293439)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCACAGGGCAGTGCGTTTAT
Hairpin Sequence:
5'-CCGG-AGCACAGGGCAGTGCGTTTAT-CTCGAG-ATAAACGCACTGCCCTGTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCACAGGGCAGTGCGTTTATCTCGAGATAAACGCACTGCCCTGTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCACAGGGCAGTGCGTTTATCTCGAGATAAACGCACTGCCCTGTGCT-3'

Other clones with same target sequence:

(none)