Construct: shRNA TRCN0000293449

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004331.2-434s21c1
DNA Barcode:
ATCATGTTTGATGTGGAAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BNIP3L (665)
Gene Description:
BCL2 interacting protein 3 like
Transcript:
RefSeq NM_004331.2 (NON-CURRENT)
Match location:
Position 434 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293449)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCATGTTTGATGTGGAAATG
Hairpin Sequence:
5'-CCGG-ATCATGTTTGATGTGGAAATG-CTCGAG-CATTTCCACATCAAACATGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCATGTTTGATGTGGAAATGCTCGAGCATTTCCACATCAAACATGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCATGTTTGATGTGGAAATGCTCGAGCATTTCCACATCAAACATGAT-3'

Other clones with same target sequence:

(none)