Construct: shRNA TRCN0000293485

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002707.3-1511s21c1
DNA Barcode:
GCTTCGGTTATTGTCATCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPM1G (5496)
Gene Description:
protein phosphatase, Mg2+/Mn2+ dependent 1G
Transcript:
RefSeq NM_002707.3 (NON-CURRENT)
Match location:
Position 1511 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293485)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCGGTTATTGTCATCCAT
Hairpin Sequence:
5'-CCGG-GCTTCGGTTATTGTCATCCAT-CTCGAG-ATGGATGACAATAACCGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCGGTTATTGTCATCCATCTCGAGATGGATGACAATAACCGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCGGTTATTGTCATCCATCTCGAGATGGATGACAATAACCGAAGC-3'

Other clones with same target sequence:

  1. TRCN0000001215