Construct: shRNA TRCN0000293609

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015436.2-379s21c1
DNA Barcode:
GGAGAATATTATTGCGATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RCHY1 (25898)
Gene Description:
ring finger and CHY zinc finger domain containing 1
Transcript:
RefSeq NM_015436.2 (NON-CURRENT)
Match location:
Position 379 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293609)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAGAATATTATTGCGATATA
Hairpin Sequence:
5'-CCGG-GGAGAATATTATTGCGATATA-CTCGAG-TATATCGCAATAATATTCTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAGAATATTATTGCGATATACTCGAGTATATCGCAATAATATTCTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAGAATATTATTGCGATATACTCGAGTATATCGCAATAATATTCTCC-3'

Other clones with same target sequence:

(none)