Construct: shRNA TRCN0000293640

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012255.3-3244s21c1
DNA Barcode:
GTGTATTCTAGATCATCTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
XRN2 (22803)
Gene Description:
5'-3' exoribonuclease 2
Transcript:
RefSeq NM_012255.3 (NON-CURRENT)
Match location:
Position 3244 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293640)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTATTCTAGATCATCTAAG
Hairpin Sequence:
5'-CCGG-GTGTATTCTAGATCATCTAAG-CTCGAG-CTTAGATGATCTAGAATACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTATTCTAGATCATCTAAGCTCGAGCTTAGATGATCTAGAATACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTATTCTAGATCATCTAAGCTCGAGCTTAGATGATCTAGAATACAC-3'

Other clones with same target sequence:

(none)