Construct: shRNA TRCN0000293696

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013285.2-2159s21c1
DNA Barcode:
GAACAGGAACATTCAAATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GNL2 (29889)
Gene Description:
G protein nucleolar 2
Transcript:
RefSeq NM_013285.2 (NON-CURRENT)
Match location:
Position 2159 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293696)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACAGGAACATTCAAATAAA
Hairpin Sequence:
5'-CCGG-GAACAGGAACATTCAAATAAA-CTCGAG-TTTATTTGAATGTTCCTGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACAGGAACATTCAAATAAACTCGAGTTTATTTGAATGTTCCTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACAGGAACATTCAAATAAACTCGAGTTTATTTGAATGTTCCTGTTC-3'

Other clones with same target sequence:

(none)