Construct: shRNA TRCN0000293708

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014353.4-427s21c1
DNA Barcode:
GCATTGACTTCCGGAACAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB26 (25837)
Gene Description:
RAB26, member RAS oncogene family
Transcript:
RefSeq NM_014353.4 (NON-CURRENT)
Match location:
Position 427 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293708)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTGACTTCCGGAACAAAG
Hairpin Sequence:
5'-CCGG-GCATTGACTTCCGGAACAAAG-CTCGAG-CTTTGTTCCGGAAGTCAATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTGACTTCCGGAACAAAGCTCGAGCTTTGTTCCGGAAGTCAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTGACTTCCGGAACAAAGCTCGAGCTTTGTTCCGGAAGTCAATGC-3'

Other clones with same target sequence:

(none)