Construct: shRNA TRCN0000293865

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198941.1-278s21c1
DNA Barcode:
GAGCACTGTCGTATCCTATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SERINC3 (10955)
Gene Description:
serine incorporator 3
Transcript:
RefSeq NM_198941.1 (NON-CURRENT)
Match location:
Position 278 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293865)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCACTGTCGTATCCTATAT
Hairpin Sequence:
5'-CCGG-GAGCACTGTCGTATCCTATAT-CTCGAG-ATATAGGATACGACAGTGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCACTGTCGTATCCTATATCTCGAGATATAGGATACGACAGTGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCACTGTCGTATCCTATATCTCGAGATATAGGATACGACAGTGCTC-3'

Other clones with same target sequence:

(none)