Construct: shRNA TRCN0000293873

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004446.2-4774s21c1
DNA Barcode:
GCCAAGTACTACACCTTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPRS1 (2058)
Gene Description:
glutamyl-prolyl-tRNA synthetase 1
Transcript:
RefSeq NM_004446.2 (NON-CURRENT)
Match location:
Position 4774 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293873)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGTACTACACCTTATTT
Hairpin Sequence:
5'-CCGG-GCCAAGTACTACACCTTATTT-CTCGAG-AAATAAGGTGTAGTACTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGTACTACACCTTATTTCTCGAGAAATAAGGTGTAGTACTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGTACTACACCTTATTTCTCGAGAAATAAGGTGTAGTACTTGGC-3'

Other clones with same target sequence:

(none)