Construct: shRNA TRCN0000293889

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018412.3-292s21c1
DNA Barcode:
GTGGTTCTTCATCGTGCTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ST7 (7982)
Gene Description:
suppression of tumorigenicity 7
Transcript:
RefSeq NM_018412.3 (NON-CURRENT)
Match location:
Position 292 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293889)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGTTCTTCATCGTGCTATT
Hairpin Sequence:
5'-CCGG-GTGGTTCTTCATCGTGCTATT-CTCGAG-AATAGCACGATGAAGAACCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGTTCTTCATCGTGCTATTCTCGAGAATAGCACGATGAAGAACCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGTTCTTCATCGTGCTATTCTCGAGAATAGCACGATGAAGAACCAC-3'

Other clones with same target sequence:

(none)