Construct: shRNA TRCN0000293913

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001237.3-2013s21c1
DNA Barcode:
TCCTAAGCAACTGGATCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCNA2 (890)
Gene Description:
cyclin A2
Transcript:
RefSeq NM_001237.3 (NON-CURRENT)
Match location:
Position 2013 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293913)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTAAGCAACTGGATCAATT
Hairpin Sequence:
5'-CCGG-TCCTAAGCAACTGGATCAATT-CTCGAG-AATTGATCCAGTTGCTTAGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTAAGCAACTGGATCAATTCTCGAGAATTGATCCAGTTGCTTAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTAAGCAACTGGATCAATTCTCGAGAATTGATCCAGTTGCTTAGGA-3'

Other clones with same target sequence:

(none)