Construct: shRNA TRCN0000293915

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031966.2-847s21c1
DNA Barcode:
TACATGACTGTCTCCATTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCNB1 (891)
Gene Description:
cyclin B1
Transcript:
RefSeq NM_031966.2 (NON-CURRENT)
Match location:
Position 847 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000293915)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACATGACTGTCTCCATTATT
Hairpin Sequence:
5'-CCGG-TACATGACTGTCTCCATTATT-CTCGAG-AATAATGGAGACAGTCATGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACATGACTGTCTCCATTATTCTCGAGAATAATGGAGACAGTCATGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACATGACTGTCTCCATTATTCTCGAGAATAATGGAGACAGTCATGTA-3'

Other clones with same target sequence:

(none)