Construct: shRNA TRCN0000294010

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017803.3-1703s21c1
DNA Barcode:
CAGATGCCGTTGAACAGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DUS2 (54920)
Gene Description:
dihydrouridine synthase 2
Transcript:
RefSeq NM_017803.3 (NON-CURRENT)
Match location:
Position 1703 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294010)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATGCCGTTGAACAGTTTG
Hairpin Sequence:
5'-CCGG-CAGATGCCGTTGAACAGTTTG-CTCGAG-CAAACTGTTCAACGGCATCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATGCCGTTGAACAGTTTGCTCGAGCAAACTGTTCAACGGCATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATGCCGTTGAACAGTTTGCTCGAGCAAACTGTTCAACGGCATCTG-3'

Other clones with same target sequence:

(none)