Construct: shRNA TRCN0000294017

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002886.2-1050s21c1
DNA Barcode:
ACGCATCCGACTCTCTAAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAP2B (5912)
Gene Description:
RAP2B, member of RAS oncogene family
Transcript:
RefSeq NM_002886.2 (NON-CURRENT)
Match location:
Position 1050 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294017)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGCATCCGACTCTCTAAATG
Hairpin Sequence:
5'-CCGG-ACGCATCCGACTCTCTAAATG-CTCGAG-CATTTAGAGAGTCGGATGCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGCATCCGACTCTCTAAATGCTCGAGCATTTAGAGAGTCGGATGCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGCATCCGACTCTCTAAATGCTCGAGCATTTAGAGAGTCGGATGCGT-3'

Other clones with same target sequence:

(none)