Construct: shRNA TRCN0000294030

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005692.3-448s21c1
DNA Barcode:
CGTTATGGCCTCATTGGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABCF2 (10061)
Gene Description:
ATP binding cassette subfamily F member 2
Transcript:
RefSeq NM_005692.3 (NON-CURRENT)
Match location:
Position 448 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294030)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTATGGCCTCATTGGTTTA
Hairpin Sequence:
5'-CCGG-CGTTATGGCCTCATTGGTTTA-CTCGAG-TAAACCAATGAGGCCATAACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTATGGCCTCATTGGTTTACTCGAGTAAACCAATGAGGCCATAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTATGGCCTCATTGGTTTACTCGAGTAAACCAATGAGGCCATAACG-3'

Other clones with same target sequence:

(none)