Construct: shRNA TRCN0000294057

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004698.2-2295s21c1
DNA Barcode:
CACTTGTTTGTGTGATCTCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRPF3 (9129)
Gene Description:
pre-mRNA processing factor 3
Transcript:
RefSeq NM_004698.2 (NON-CURRENT)
Match location:
Position 2295 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294057)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTTGTTTGTGTGATCTCAG
Hairpin Sequence:
5'-CCGG-CACTTGTTTGTGTGATCTCAG-CTCGAG-CTGAGATCACACAAACAAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTTGTTTGTGTGATCTCAGCTCGAGCTGAGATCACACAAACAAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTTGTTTGTGTGATCTCAGCTCGAGCTGAGATCACACAAACAAGTG-3'

Other clones with same target sequence:

(none)