Construct: shRNA TRCN0000294063

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001662.2-125s21c1
DNA Barcode:
AGACCACAATCCTGTACAAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARF5 (381)
Gene Description:
ADP ribosylation factor 5
Transcript:
RefSeq NM_001662.2 (NON-CURRENT)
Match location:
Position 125 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294063)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACCACAATCCTGTACAAAC
Hairpin Sequence:
5'-CCGG-AGACCACAATCCTGTACAAAC-CTCGAG-GTTTGTACAGGATTGTGGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACCACAATCCTGTACAAACCTCGAGGTTTGTACAGGATTGTGGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACCACAATCCTGTACAAACCTCGAGGTTTGTACAGGATTGTGGTCT-3'

Other clones with same target sequence:

(none)