Construct: shRNA TRCN0000294082

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144677.2-1585s21c1
DNA Barcode:
AGCAGTTCATGACCATGTTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MGAT5B (146664)
Gene Description:
alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase B
Transcript:
RefSeq NM_144677.2 (NON-CURRENT)
Match location:
Position 1585 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294082)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAGTTCATGACCATGTTTC
Hairpin Sequence:
5'-CCGG-AGCAGTTCATGACCATGTTTC-CTCGAG-GAAACATGGTCATGAACTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAGTTCATGACCATGTTTCCTCGAGGAAACATGGTCATGAACTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAGTTCATGACCATGTTTCCTCGAGGAAACATGGTCATGAACTGCT-3'

Other clones with same target sequence:

(none)