Construct: shRNA TRCN0000294099

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022118.3-2993s21c1
DNA Barcode:
TACCTGTGTTTCATTAGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBM26 (64062)
Gene Description:
RNA binding motif protein 26
Transcript:
RefSeq NM_022118.3 (NON-CURRENT)
Match location:
Position 2993 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294099)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACCTGTGTTTCATTAGTATT
Hairpin Sequence:
5'-CCGG-TACCTGTGTTTCATTAGTATT-CTCGAG-AATACTAATGAAACACAGGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACCTGTGTTTCATTAGTATTCTCGAGAATACTAATGAAACACAGGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACCTGTGTTTCATTAGTATTCTCGAGAATACTAATGAAACACAGGTA-3'

Other clones with same target sequence:

  1. TRCN0000305476