Construct: shRNA TRCN0000294109

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021239.2-981s21c1
DNA Barcode:
TCATCACTAAGGAGGATATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBM25 (58517)
Gene Description:
RNA binding motif protein 25
Transcript:
RefSeq NM_021239.2 (NON-CURRENT)
Match location:
Position 981 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294109)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATCACTAAGGAGGATATAA
Hairpin Sequence:
5'-CCGG-TCATCACTAAGGAGGATATAA-CTCGAG-TTATATCCTCCTTAGTGATGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATCACTAAGGAGGATATAACTCGAGTTATATCCTCCTTAGTGATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATCACTAAGGAGGATATAACTCGAGTTATATCCTCCTTAGTGATGA-3'

Other clones with same target sequence:

(none)