Construct: shRNA TRCN0000294302

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020424.3-330s21c1
DNA Barcode:
TTGGCCTCTACCGCAGCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LYRM1 (57149)
Gene Description:
LYR motif containing 1
Transcript:
RefSeq NM_020424.3 (NON-CURRENT)
Match location:
Position 330 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294302)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGCCTCTACCGCAGCATTT
Hairpin Sequence:
5'-CCGG-TTGGCCTCTACCGCAGCATTT-CTCGAG-AAATGCTGCGGTAGAGGCCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGCCTCTACCGCAGCATTTCTCGAGAAATGCTGCGGTAGAGGCCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGCCTCTACCGCAGCATTTCTCGAGAAATGCTGCGGTAGAGGCCAA-3'

Other clones with same target sequence:

(none)