Construct: shRNA TRCN0000294328

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004392.4-1358s21c1
DNA Barcode:
TCATGTCTCCTGGGATAATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DACH1 (1602)
Gene Description:
dachshund family transcription factor 1
Transcript:
RefSeq NM_004392.4 (NON-CURRENT)
Match location:
Position 1358 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294328)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATGTCTCCTGGGATAATTC
Hairpin Sequence:
5'-CCGG-TCATGTCTCCTGGGATAATTC-CTCGAG-GAATTATCCCAGGAGACATGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATGTCTCCTGGGATAATTCCTCGAGGAATTATCCCAGGAGACATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATGTCTCCTGGGATAATTCCTCGAGGAATTATCCCAGGAGACATGA-3'

Other clones with same target sequence:

(none)