Construct: shRNA TRCN0000294357

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152999.3-1035s21c1
DNA Barcode:
GCCAGTGGTGGTAGCTATAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STEAP2 (261729)
Gene Description:
STEAP2 metalloreductase
Transcript:
RefSeq NM_152999.3 (NON-CURRENT)
Match location:
Position 1035 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294357)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGTGGTGGTAGCTATAAG
Hairpin Sequence:
5'-CCGG-GCCAGTGGTGGTAGCTATAAG-CTCGAG-CTTATAGCTACCACCACTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGTGGTGGTAGCTATAAGCTCGAGCTTATAGCTACCACCACTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGTGGTGGTAGCTATAAGCTCGAGCTTATAGCTACCACCACTGGC-3'

Other clones with same target sequence:

(none)