Construct: shRNA TRCN0000294375

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000963.2-1487s21c1
DNA Barcode:
TGAGTACCGCAAACGCTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTGS2 (5743)
Gene Description:
prostaglandin-endoperoxide synthase 2
Transcript:
RefSeq NM_000963.2 (NON-CURRENT)
Match location:
Position 1487 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294375)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAGTACCGCAAACGCTTTAT
Hairpin Sequence:
5'-CCGG-TGAGTACCGCAAACGCTTTAT-CTCGAG-ATAAAGCGTTTGCGGTACTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGTACCGCAAACGCTTTATCTCGAGATAAAGCGTTTGCGGTACTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGTACCGCAAACGCTTTATCTCGAGATAAAGCGTTTGCGGTACTCA-3'

Other clones with same target sequence:

(none)