Construct: shRNA TRCN0000294413

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152699.4-2631s21c1
DNA Barcode:
CACTATTGTTACCTCAAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SENP5 (205564)
Gene Description:
SUMO specific peptidase 5
Transcript:
RefSeq NM_152699.4 (NON-CURRENT)
Match location:
Position 2631 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294413)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTATTGTTACCTCAAATTT
Hairpin Sequence:
5'-CCGG-CACTATTGTTACCTCAAATTT-CTCGAG-AAATTTGAGGTAACAATAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTATTGTTACCTCAAATTTCTCGAGAAATTTGAGGTAACAATAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTATTGTTACCTCAAATTTCTCGAGAAATTTGAGGTAACAATAGTG-3'

Other clones with same target sequence:

(none)