Construct: shRNA TRCN0000294439

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014405.2-1079s21c1
DNA Barcode:
GACGACGAACAATGAACTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CACNG4 (27092)
Gene Description:
calcium voltage-gated channel auxiliary subunit gamma 4
Transcript:
RefSeq NM_014405.2 (NON-CURRENT)
Match location:
Position 1079 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294439)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGACGAACAATGAACTAAA
Hairpin Sequence:
5'-CCGG-GACGACGAACAATGAACTAAA-CTCGAG-TTTAGTTCATTGTTCGTCGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGACGAACAATGAACTAAACTCGAGTTTAGTTCATTGTTCGTCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGACGAACAATGAACTAAACTCGAGTTTAGTTCATTGTTCGTCGTC-3'

Other clones with same target sequence:

(none)