Construct: shRNA TRCN0000294440

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006321.2-1119s21c1
DNA Barcode:
CTGTGCCACAATCCGGAAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARIH2 (10425)
Gene Description:
ariadne RBR E3 ubiquitin protein ligase 2
Transcript:
RefSeq NM_006321.2 (NON-CURRENT)
Match location:
Position 1119 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294440)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTGCCACAATCCGGAAATG
Hairpin Sequence:
5'-CCGG-CTGTGCCACAATCCGGAAATG-CTCGAG-CATTTCCGGATTGTGGCACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTGCCACAATCCGGAAATGCTCGAGCATTTCCGGATTGTGGCACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTGCCACAATCCGGAAATGCTCGAGCATTTCCGGATTGTGGCACAG-3'

Other clones with same target sequence:

(none)