Construct: shRNA TRCN0000294476

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004576.2-1878s21c1
DNA Barcode:
TAATCTCACATACTGAATACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP2R2B (5521)
Gene Description:
protein phosphatase 2 regulatory subunit Bbeta
Transcript:
RefSeq NM_004576.2 (NON-CURRENT)
Match location:
Position 1878 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294476)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAATCTCACATACTGAATACT
Hairpin Sequence:
5'-CCGG-TAATCTCACATACTGAATACT-CTCGAG-AGTATTCAGTATGTGAGATTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAATCTCACATACTGAATACTCTCGAGAGTATTCAGTATGTGAGATTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAATCTCACATACTGAATACTCTCGAGAGTATTCAGTATGTGAGATTA-3'

Other clones with same target sequence:

  1. TRCN0000009862