Construct: shRNA TRCN0000294504

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133998.3-321s21c1
DNA Barcode:
GTGCAGAGCCAAAGGCCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam207a (108707)
Gene Description:
family with sequence similarity 207, member A
Transcript:
RefSeq NM_133998.3 (CURRENT)
Match location:
Position 321 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294504)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCAGAGCCAAAGGCCATTT
Hairpin Sequence:
5'-CCGG-GTGCAGAGCCAAAGGCCATTT-CTCGAG-AAATGGCCTTTGGCTCTGCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCAGAGCCAAAGGCCATTTCTCGAGAAATGGCCTTTGGCTCTGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCAGAGCCAAAGGCCATTTCTCGAGAAATGGCCTTTGGCTCTGCAC-3'

Other clones with same target sequence:

(none)