Construct: shRNA TRCN0000294514

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017374.3-347s21c1
DNA Barcode:
GAACGAGTGTAAGCAGCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp2cb (19053)
Gene Description:
protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform
Transcript:
RefSeq NM_017374.3 (CURRENT)
Match location:
Position 347 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294514)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACGAGTGTAAGCAGCTGAA
Hairpin Sequence:
5'-CCGG-GAACGAGTGTAAGCAGCTGAA-CTCGAG-TTCAGCTGCTTACACTCGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACGAGTGTAAGCAGCTGAACTCGAGTTCAGCTGCTTACACTCGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACGAGTGTAAGCAGCTGAACTCGAGTTCAGCTGCTTACACTCGTTC-3'

Other clones with same target sequence:

(none)