Construct: shRNA TRCN0000294583

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001142959.1-267s21c1
DNA Barcode:
GCTCAGGAGAGGATTACTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bcl2l15 (229672)
Gene Description:
BCLl2-like 15
Transcript:
RefSeq NM_001142959.1 (CURRENT)
Match location:
Position 267 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294583)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAGGAGAGGATTACTCTT
Hairpin Sequence:
5'-CCGG-GCTCAGGAGAGGATTACTCTT-CTCGAG-AAGAGTAATCCTCTCCTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAGGAGAGGATTACTCTTCTCGAGAAGAGTAATCCTCTCCTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAGGAGAGGATTACTCTTCTCGAGAAGAGTAATCCTCTCCTGAGC-3'

Other clones with same target sequence:

  1. TRCN0000009725