Construct: shRNA TRCN0000294589
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_145933.3-1897s21c1
- DNA Barcode:
- CGCTCCTCTTCGAGAAGAATA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- St6gal1 (20440)
- Gene Description:
- beta galactoside alpha 2,6 sialyltransferase 1
- Transcript:
- RefSeq NM_145933.3 (NON-CURRENT)
- Match location:
- Position 1897 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000294589)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGCTCCTCTTCGAGAAGAATA
- Hairpin Sequence:
-
5'-CCGG-CGCTCCTCTTCGAGAAGAATA-CTCGAG-TATTCTTCTCGAAGAGGAGCG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGCTCCTCTTCGAGAAGAATACTCGAGTATTCTTCTCGAAGAGGAGCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGCTCCTCTTCGAGAAGAATACTCGAGTATTCTTCTCGAAGAGGAGCG-3'
Other clones with same target sequence:
-
TRCN0000018818
