Construct: shRNA TRCN0000294602

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009371.2-2287s21c1
DNA Barcode:
CTCAGGAAATGAGATTGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tgfbr2 (21813)
Gene Description:
transforming growth factor, beta receptor II
Transcript:
RefSeq NM_009371.2 (NON-CURRENT)
Match location:
Position 2287 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294602)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAGGAAATGAGATTGATTT
Hairpin Sequence:
5'-CCGG-CTCAGGAAATGAGATTGATTT-CTCGAG-AAATCAATCTCATTTCCTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAGGAAATGAGATTGATTTCTCGAGAAATCAATCTCATTTCCTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAGGAAATGAGATTGATTTCTCGAGAAATCAATCTCATTTCCTGAG-3'

Other clones with same target sequence:

(none)