Construct: shRNA TRCN0000294607

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019827.6-2613s21c1
DNA Barcode:
CCACTCAAGAACTGTCAAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gsk3b (56637)
Gene Description:
glycogen synthase kinase 3 beta
Transcript:
RefSeq NM_019827.6 (NON-CURRENT)
Match location:
Position 2613 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294607)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTCAAGAACTGTCAAGTA
Hairpin Sequence:
5'-CCGG-CCACTCAAGAACTGTCAAGTA-CTCGAG-TACTTGACAGTTCTTGAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTCAAGAACTGTCAAGTACTCGAGTACTTGACAGTTCTTGAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTCAAGAACTGTCAAGTACTCGAGTACTTGACAGTTCTTGAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000012614