Construct: shRNA TRCN0000294676

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025845.2-1908s21c1
DNA Barcode:
GATCACATTGAATCCTATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prpf38b (66921)
Gene Description:
PRP38 pre-mRNA processing factor 38 (yeast) domain containing B
Transcript:
RefSeq NM_025845.2 (CURRENT)
Match location:
Position 1908 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294676)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCACATTGAATCCTATAAA
Hairpin Sequence:
5'-CCGG-GATCACATTGAATCCTATAAA-CTCGAG-TTTATAGGATTCAATGTGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCACATTGAATCCTATAAACTCGAGTTTATAGGATTCAATGTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCACATTGAATCCTATAAACTCGAGTTTATAGGATTCAATGTGATC-3'

Other clones with same target sequence:

(none)