Construct: shRNA TRCN0000294677

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016690.4-1397s21c1
DNA Barcode:
TGTTGACCTAAGATGATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hnrnpdl (50926)
Gene Description:
heterogeneous nuclear ribonucleoprotein D-like
Transcript:
RefSeq NM_016690.4 (CURRENT)
Match location:
Position 1397 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294677)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTTGACCTAAGATGATTATT
Hairpin Sequence:
5'-CCGG-TGTTGACCTAAGATGATTATT-CTCGAG-AATAATCATCTTAGGTCAACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTTGACCTAAGATGATTATTCTCGAGAATAATCATCTTAGGTCAACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTTGACCTAAGATGATTATTCTCGAGAATAATCATCTTAGGTCAACA-3'

Other clones with same target sequence:

(none)