Construct: shRNA TRCN0000294706

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133242.2-705s21c1
DNA Barcode:
GATTGCCTCATAGCATCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbm39 (170791)
Gene Description:
RNA binding motif protein 39
Transcript:
RefSeq NM_133242.2 (NON-CURRENT)
Match location:
Position 705 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294706)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTGCCTCATAGCATCAAAT
Hairpin Sequence:
5'-CCGG-GATTGCCTCATAGCATCAAAT-CTCGAG-ATTTGATGCTATGAGGCAATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTGCCTCATAGCATCAAATCTCGAGATTTGATGCTATGAGGCAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTGCCTCATAGCATCAAATCTCGAGATTTGATGCTATGAGGCAATC-3'

Other clones with same target sequence:

(none)