Construct: shRNA TRCN0000294810

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016813.2-1959s21c1
DNA Barcode:
GATTCCAGAAGTAGCGTTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nxf1 (53319)
Gene Description:
nuclear RNA export factor 1
Transcript:
RefSeq NM_016813.2 (CURRENT)
Match location:
Position 1959 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294810)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTCCAGAAGTAGCGTTTAT
Hairpin Sequence:
5'-CCGG-GATTCCAGAAGTAGCGTTTAT-CTCGAG-ATAAACGCTACTTCTGGAATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTCCAGAAGTAGCGTTTATCTCGAGATAAACGCTACTTCTGGAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTCCAGAAGTAGCGTTTATCTCGAGATAAACGCTACTTCTGGAATC-3'

Other clones with same target sequence:

(none)