Construct: shRNA TRCN0000294913

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023735.1-1245s21c1
DNA Barcode:
GCCTATTGATGTACAAGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Actr3 (74117)
Gene Description:
ARP3 actin-related protein 3
Transcript:
RefSeq NM_023735.1 (NON-CURRENT)
Match location:
Position 1245 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294913)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTATTGATGTACAAGTTAT
Hairpin Sequence:
5'-CCGG-GCCTATTGATGTACAAGTTAT-CTCGAG-ATAACTTGTACATCAATAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTATTGATGTACAAGTTATCTCGAGATAACTTGTACATCAATAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTATTGATGTACAAGTTATCTCGAGATAACTTGTACATCAATAGGC-3'

Other clones with same target sequence:

(none)