Construct: shRNA TRCN0000294915
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_010860.3-473s21c1
- DNA Barcode:
- ACATTCTGTATCCCGAGTCTG
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Myl6 (17904)
- Gene Description:
- myosin, light polypeptide 6, alkali, smooth muscle and non-muscle
- Transcript:
- RefSeq NM_010860.3 (NON-CURRENT)
- Match location:
- Position 473 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000294915)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- ACATTCTGTATCCCGAGTCTG
- Hairpin Sequence:
-
5'-CCGG-ACATTCTGTATCCCGAGTCTG-CTCGAG-CAGACTCGGGATACAGAATGT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGACATTCTGTATCCCGAGTCTGCTCGAGCAGACTCGGGATACAGAATGTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAACATTCTGTATCCCGAGTCTGCTCGAGCAGACTCGGGATACAGAATGT-3'
Other clones with same target sequence:
(none)
