Construct: shRNA TRCN0000294963

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133966.2-2317s21c1
DNA Barcode:
ACTCCTGGCACTGGCTATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Taf5l (102162)
Gene Description:
TATA-box binding protein associated factor 5 like
Transcript:
RefSeq NM_133966.2 (CURRENT)
Match location:
Position 2317 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294963)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCCTGGCACTGGCTATAAT
Hairpin Sequence:
5'-CCGG-ACTCCTGGCACTGGCTATAAT-CTCGAG-ATTATAGCCAGTGCCAGGAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCCTGGCACTGGCTATAATCTCGAGATTATAGCCAGTGCCAGGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCCTGGCACTGGCTATAATCTCGAGATTATAGCCAGTGCCAGGAGT-3'

Other clones with same target sequence:

(none)