Construct: shRNA TRCN0000295035

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144802.3-1261s21c1
DNA Barcode:
GACTACACTAAACCTTATTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hnrnpll (72692)
Gene Description:
heterogeneous nuclear ribonucleoprotein L-like
Transcript:
RefSeq NM_144802.3 (NON-CURRENT)
Match location:
Position 1261 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295035)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTACACTAAACCTTATTTG
Hairpin Sequence:
5'-CCGG-GACTACACTAAACCTTATTTG-CTCGAG-CAAATAAGGTTTAGTGTAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTACACTAAACCTTATTTGCTCGAGCAAATAAGGTTTAGTGTAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTACACTAAACCTTATTTGCTCGAGCAAATAAGGTTTAGTGTAGTC-3'

Other clones with same target sequence:

(none)