Construct: shRNA TRCN0000295102

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_148930.2-2820s21c1
DNA Barcode:
CTAAGGTGTATATAGTGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbm5 (83486)
Gene Description:
RNA binding motif protein 5
Transcript:
RefSeq NM_148930.2 (NON-CURRENT)
Match location:
Position 2820 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295102)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAAGGTGTATATAGTGTAAA
Hairpin Sequence:
5'-CCGG-CTAAGGTGTATATAGTGTAAA-CTCGAG-TTTACACTATATACACCTTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAAGGTGTATATAGTGTAAACTCGAGTTTACACTATATACACCTTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAAGGTGTATATAGTGTAAACTCGAGTTTACACTATATACACCTTAG-3'

Other clones with same target sequence:

(none)