Construct: shRNA TRCN0000295258

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018884.1-904s21c1
DNA Barcode:
CAAATTCATGACAGGATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pdzrn3 (55983)
Gene Description:
PDZ domain containing RING finger 3
Transcript:
RefSeq NM_018884.1 (NON-CURRENT)
Match location:
Position 904 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295258)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAATTCATGACAGGATTATT
Hairpin Sequence:
5'-CCGG-CAAATTCATGACAGGATTATT-CTCGAG-AATAATCCTGTCATGAATTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAATTCATGACAGGATTATTCTCGAGAATAATCCTGTCATGAATTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAATTCATGACAGGATTATTCTCGAGAATAATCCTGTCATGAATTTG-3'

Other clones with same target sequence:

(none)