Construct: shRNA TRCN0000295266

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177462.4-1916s21c1
DNA Barcode:
TAGCTACAACACCAGTTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zmym6 (100177)
Gene Description:
zinc finger, MYM-type 6
Transcript:
RefSeq NM_177462.4 (NON-CURRENT)
Match location:
Position 1916 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295266)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGCTACAACACCAGTTATAA
Hairpin Sequence:
5'-CCGG-TAGCTACAACACCAGTTATAA-CTCGAG-TTATAACTGGTGTTGTAGCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGCTACAACACCAGTTATAACTCGAGTTATAACTGGTGTTGTAGCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGCTACAACACCAGTTATAACTCGAGTTATAACTGGTGTTGTAGCTA-3'

Other clones with same target sequence:

(none)