Construct: shRNA TRCN0000295277

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177462.4-755s21c1
DNA Barcode:
AGCCTGTTGTTACCATATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zmym6 (100177)
Gene Description:
zinc finger, MYM-type 6
Transcript:
RefSeq NM_177462.4 (NON-CURRENT)
Match location:
Position 755 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295277)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCCTGTTGTTACCATATATA
Hairpin Sequence:
5'-CCGG-AGCCTGTTGTTACCATATATA-CTCGAG-TATATATGGTAACAACAGGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCCTGTTGTTACCATATATACTCGAGTATATATGGTAACAACAGGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCCTGTTGTTACCATATATACTCGAGTATATATGGTAACAACAGGCT-3'

Other clones with same target sequence:

(none)